Journal: eLife

Article Title: Enzyme activity and selectivity filter stability of ancient TRPM2 channels were simultaneously lost in early vertebrates

doi: 10.7554/eLife.44556

Figure Lengend Snippet:

Article Snippet: Gene ( Salpingoeca rosetta TRPM2) , srTRPM2, srNUDT9-H , General Biosystems, Inc. , NCBI: XP_004993318 , species-optimized synthetic genes.

Techniques: Plasmid Preparation, Mutagenesis, Avidin-Biotin Assay, Software

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: ( a ) Phylogenetic tree constructed by the neighbor-joining method with amino acid sequences of L01 and reported laccases. ( b ) Structure model of L01 by SWISS-MODEL based on the amino sequence.

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: Construct, Sequencing

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: Multiple sequence alignments of L01 with other well-characterized bacterial and fungal laccases, with conserved motifs marked in blue boxes.

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: Sequencing

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: SDS-PAGE analysis of L01. Samples were loaded in different lanes. Lane M, prestained protein ladder; Lanes 1–2, the concentrated fermentation broth; Lanes 3–4, purified L01.

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: SDS Page, Purification

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: ( a ) Effect of temperature on L01 catalytic activity; ( b ) effect of temperature on L01 stability after 2 h of incubation ( n = 3).

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: Activity Assay, Incubation

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: ( a ) L01 catalytic activity at different pH; ( b ) L01 stability at different pH after 4 h of incubation ( n = 3).

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: Activity Assay, Incubation

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: Comparison of properties between L01 and reported laccases.

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: Comparison

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: Effects of metal ions, SDS, TW, ME, and EDTA on L01 activity ( n = 3). TW: Tween 80, ME: mercaptoethanol.

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: Activity Assay

Journal: Molecules

Article Title: Characterization of a New Laccase from Vibrio sp. with pH-stability, Salt-tolerance, and Decolorization Ability

doi: 10.3390/molecules28073037

Figure Lengend Snippet: Decolorization rates (%) of synthetic dyes by L01 and commercial laccase ( n = 3).

Article Snippet: We entrusted Synbio Technologies (Suzhou, China) to synthesize the gene L01 with the XPR2 signal peptide after codons were optimized, and then transformed the obtained DNA fragment into a URA-strain [ ].

Techniques: